Glycoprofiling of Oligosaccharides of Regular and Lactose-Free Milk by Mass Spectrometry

Oligosaccharides from regular milk and lactose-free were analyzed by Electrospray Ionization interface coupled with Ion Trap Mass Spectrometry (IT-MS). The negative mode mass spectrometry of sugar compositions was obtained either direct infusion Liquid Chromatography (LC). Hexose (Hex) the other oligomeric components observed in both milk. While lactose derivatives (deprotonated dimer, chloride adducts, dimer) have seen to be dominated, monosaccharide (Hex-H2O, Hex, Hex-Cl) as abundant which lytic product lactose. Phosphate/sulfate ester substitution on sialyllactose samples similar intensity thus it has been understood that these important are not digested during removing process.